I wanna bang my head!!!!
I wanna bang my head!!!!
BANG BANG BANG!
Stupid me, dunno how to distinguish Hypersensitivity!.
Omg!!.
BANG more!.
Da Tou really empty-minded.

Wanna take a short nap after paper,
but the qn, the Qn!!.
Made me feel so bad and suffocated.
The rest said that if rest of question I know how to do,
I should be fine.
But I feel so wasted.
So wasted!.
I may get better grade, maybe C instead of D?.

Can I BANG my head again?!.
BANG BANG!.
T.T
Wanna cry but can't cry out..
It hurts till... I can't describe.
And yah...
I gotta overcome myself.
A step to move on...
I need counseling -.-
I just feel dumb la!.
I can't believe my effort is gone just like that!.
But plead God will be merciful to grant me a PASS kks?.
It'll be more than enough for my case now.

I had to lie to myself..
And smth funny, I feel it more miserable failing a paper than not been able to get a bf!.
Hahas. I managed to laugh at myself for a while then return back to emo state.
But what's wrong with me,
I shouldn't let all these affect me.
Freak me.

Ks, things that satisfy me today:
Fruit tart
Blouse or u can call it a balloon shirt(???..)
Short
All black except the tart.
The tart is colorful,
I hope my mood can be that color now..
Bless me pls.
Just some will do.
I'm not greedy.

BANG WALL BANG WALL.

Hais..
Still in the midst of struggling.

Memorized vaccine development not long ago,
so yah what I can recall is Recombinant DNA vaccine
(e.g. Hepatitis B vaccine),
DNA vaccine, whereby plasmid DNA encodes an immunogen
and injected subcutaneously, synthetic vaccine,
an immunogenic peptide that is able to mount a protective immune reponse,
and virus-carrier vaccine,
using live virus such as poliovirus by means of a vector,
then what happens when virus enters into host cell?.

Wahahas let Miss Lim tell you...
Virus will construct replicates in the host,
express it's foreign genes which serve as a vaccine to that particular antigen.

Pardon?. to which antigen?.
Don't ask me, ask the notes.
I just memorize it blindly XP
Haisya.. Bless me pls bless me!.

Sick and dumb and yes so unlucky to have come to Biotech and suffer like hell.
But nvm, if I pass my exam, it's worth it all!.


Cheers Manda & Cheers Everybody!.

Hais..
Still in the midst of struggling.
Memorized vaccine development not long ago, so yah
what I can recall is Recombinant DNA vaccine (e.g. Hepatitis B vaccine),
DNA vaccine, whereby plasmid DNA encodes an immunogen and injected subcutaneously,
synthetic vaccine, an immunogenic peptide that is able to mount a protective immune reponse,
and virus-carrier vaccine, using live virus such as poliovirus by means of a vector,
then what happens when virus enters into host cell?.
Wahahas let Miss Lim tell you...
Virus will construct replicates in the host, express it's foreign genes which serve as a vaccine to that particular antigen.
Pardon?. to which antigen?.
Don't ask me, ask the notes. I just memorize it blindly XP
Haisya..
Bless me pls bless me!.
Sick and dumb and yes so unlucky to have come to Biotech and suffer like hell.
But nvm, if I pass my exam, it's worth it all!.
Cheers Manda

&

Cheers
Everybody!.

abc
Production of plants with prolonged shelf life using direct gene transfer method:
1. Generate plasmid(Ti/Ri) containing genes for extended shelf-life.
- Plasmid can then be transferred into plant cells by various direct gene transfer method.
2.Liposomes/ synthetic lipid vesicles:
-liposomes allow entrapment of DNA and can interact with a variety of cells
-plasmids will eventually be transferred to their nucleus
-liposomes protect foreign DNA contained inside from nuclease enzyme attack
3. PEG+Ca2t+high pH
-for direct gene transfer to dicot and monocot protoplast
-with PEG at high pH in presence of ca2+
-for delivery of naked DNA or DNA encapsulated in liposomes
-increases permeability of cell membrane to DNA
-stable integration of introduced DNA
-regeneration of transformed plants
4.Electroporation
-application of low A.C supply and high D.C supply
-induce transient pore formation in plasma membrane for entry of DNA
-time taken is critical for DNA transfer and cell survival
-optimum conditions
-foreign gene expression achieved in intact bacterial, yeast and etc.
-as well as protoplasts of fungi and plants
5. Microprojectile bombardment
-using high velocity DNA-coated microprojectile
-exogenous DNA or RNA can be coated on surface of microscopic tungsten/gold microprojectiles
-then accelerated to high velocities by a particle gun to pierce walls and membranes
6.Microinjections
-using capillary micropipettes
-most direct and precise method of delivering DNA into specific cell compartments
-but requires sophisticated euipment and well-trained personnel

This question is worth of 15 marks.
15 marks!.
Omg.. I hope my answer to this is roughly there.
Ks, this is just for practice and understanding.
Gonna memorise soon.
Biotech = memorise = so died

Done =)
will be back soon!.

abc
WOoohOooo...
Wake up early these days,
just to study for exam T.T
But nvm,
if I manage to pass all,
it's really worth it.
And umpteen times I keep telling myself,
"if I fail this semestral exam, I will drop out"
But don't you find me being contradictive?.
I mean I know I'll miss my friends there,
then why I say all these?.
As if I'll really do -.-
Hais.

6 meis so pissed off by me yesterday.
lol.
I'm so sorry gal.
I'm optimistic,
but not to my situation.
Whatever, just ignore me. lol.

Yeas peeps,
I'm really studying very hard.
First time,
each and every single day,
I'm concentrating and building up my confidence and morality.
Hahas. Hopefully it helps =)

I must believe I can, yes Manda can can!.
lol.
Hais.
Then Tony called yesterday,
asked if I'm in Clarke Quay,
and that triggered my thoughts for Grill.
Hais. I miss there so much so much.
A place where Manda change to be an independent ass.
lol.
Ks, I shall visit Sam and Chong and Aunty Kwan and Sotong Jie one day after exam =)

Hokay,
basically I've been studying these days and I'm sick too.
Sore throat+ flu+ cough + ulcer.
And it's really disturbing when you're studying.
Think my body can't handle the stress.
As long I'm mentally prepared,
I'm on my way!.

Ks, cheers everyone.
And stay away from ME.
lol.
Don't wanna spread my germs to ya guys T.T
Drink more water and take vitamin C if necessary!.

Love,
to All

abc

Feels so tired.
But I won't stop.
I keep on reminding myself,
it's barely 2 weeks,
after this,
the day will welcome us with rainbows again =)

Manda ar Manda...
You can do it!.
Trust yourself.
You are stupid but you still manage to survive through so much obstacles.
You won't die so easily!.

Recently, I'm so disturbed over certain stuffs.
I wanna help but I shouldn't interfere.
And it's not outsiders whom can make comments to...
Anyway, I gotta wash my hands off these.
What I can do is to accompany, share and care.

Hais.
Can't sleep last night.
Why am I so bothered by peeps around me,
and hence I realized I neglect myself.
But isn't that the purpose I'm living for?.
To help and care...
Pls god, if you do exist,
do give me lotsa strengths,
so that I can help others and myself.
And too, I realized I'm getting a bit annoyed,
but it's my fault.
I shouldn't have thrown my temper onto them.
Instead, it should be the problem itself.
Manda, you gotta know how to deal with these situation!.
Grow tough!.
Jia you...

Cheers & Love,
Da Tou

Halo everybody =D
Suddenly dunno what to talk about. lol.
Hokay, let me talk bout thursday!.
The day when I really enjoy myself,
no work, only them =)

In the afternoon bout 230pm, we met at usual bus stop.
The we refers to 2 Jie, 6 Meis and me =)
Both babes were super pretty!. Wore dress and black cardigans.
As for me, totally no mood to dress up myself,
end up wearing T-shirts and shorts.
Like going market like that.
lol.
Manda-aunty!!...

We then went to Tampines mall to have Pasta Mania!!.
Mama mia!.
We called for combos and sad to say,
the garlic breads disappoint us.
But nvm, the rather long chit chats we had managed to compensate our disappointment. lol.
Iya, gals ar, we forgot to take photos of the food!. -.-

Realised that some people is taking advantage,
so yah,
watch out man.

Hokay, then we met Tony and his fren, Wei Xiong in Funan IT mall.
Hahahs.
His friend so funny lah!.

After we had Anderson's Ice cream,
we stood outside Funan and got frantic over the fireworks.
lol. But think it's me alone lah.
I'm siao za bo sia.
In addition, I hope you guys know that the day I'm evaluating is National Day!.
Hahahas!!.

We waited for 1 Jie and Jie fu,
then proceed on to a pub, blue blazze at Boat Quay. (Thanks to the blogs from Jie and Meis, that I know the name of the pub)
lol!.
Yeah!. Then Wei Xiong treated us to his Martel!.
So we had Martel+ green tea =D
Had beef hor fun for dinner to =)
Then we played games and sing KTV!.
WOooots!.
Hao and rest know that I'm a lousy drinker, so kept diluting mine with green tea.
lol.
Thanks u gals =)

Think I had too much or what,
I felt sleepy and started to rest on the sofa.
Of course I'm visually and mentally clear of my surrounding la.
The rest thought I'm drunk and had fell aslept.
I saw them chatting near the toilet and thanks goodness my eyes were small,
I then spy them for a while lo XP
Later 6 meis fu came to talk to me.
Yeah, had so much enlightenment from him,
thanks u meis fu =)
And yeah, manda-aka-6 meis is really fine le =D

In 6 meis's blog, she mentioned bout meis fu like ji kor bei,
omg!!.. really laugh out loud lah!.
Hahahahs!.
And btw, I not ding dong bell lo!..
I'm really alert lah!!.
lol.
And meis ar, not all guys having that honest look means that they are nice guys lo.
I'm not interested, but yah, did notice him only mah -.-
Hahas.
Anyway, the day is fun and I love ya guys a lot!.

Thanks to:
7 jie meis
Mich
Ruby
Weewee
Ish
Huiyi
Kiwi
Kok
Ms Ang
Cindy
Gim

for giving me motivations to work on.
Yessa!!..
I will jia you de =D

Cheers
Da Tou

Feels weird today.
Like not me at all.
Lol.
Yah, went to school for an hour, came back home,
and gonna skip Karate ltr.
Hais.
I need to prioritize and of cos, exam comes first.
No use going for Karate cos I might leave the school anytime if things ain't right for me.
I love karate..
I wanna participate in the grading this year...
But somehow, lots more things are challenging my time and tolerance.
What can I do.
Yah.. Hao is right, what if god don't exist.
Whatever happens, is my mindset lo.
Nvm, I will work hard.
I won't surrender to obstacles.
Come on, just small matters!.
Manda can surely do it!.
Some things can't be solved,
not that I wanna avoid them,
but instead they avoid me!.
What can I do about it?.
To chase them around like nuts?.
No way man.
Or I'll not be Manda.
I'm born to be tough, to be like a man,
to be brotherly to all.
So be it, I don't mind =D

Besides that, I wanna apologize to my 1 Jie and 6 Meis for causing ya gals to worry for me again.
I don't want this to happen again.
I want ya gals to be reassured that I'll be fine no matter what.
Be it single, double, triple... lol.
I'll still be solid.
Things can be flexible de mah, if you are able to think through thoroughly,
you're still yourself and shows that I've grown up a bit more!. =)

To certain things I may know nuts.
But come to life, I learned alot and yessa,
I accept all.
I'm born like this,
I respect myself for being like this lo.
But that doesn't mean I don't change to be a better person la.

Everyone needs to die,
why don't I live and get the taste of life,
and hence no regret when I'm not on earth anymore =)

Yessa!. Cheer up Manda and peeps who are not feeling that good.
Come on, we can pull through all.

Cheers,
Study hard!.

Actually I'm not sad anymore..
But when I come across alot of things,
I'm feel so depressed.

A question to god,
"Why are you so cruel to me?."
"Had I done anything sinful in my past life that you ought to punish me emotionally?"

I know there are always ups and downs in life,
but why obstacles kept rushing towards me...
I don't want to be an unhappy person T.T
I can't help to be sad, I can only try to suppress my feelings.
To be strong, I smile and laugh every single day.

The day when he replied to my msg,
I was like wth.
Why such person would have entered into my life again.

I don't need sympathize,
I just want honest peeps.
No guys.
I'd rather know gays duh.
Cos at least they are true right there in the heart.

If you aren't true there,
how to live happily ever after?.
If you love the person with no commitments,
what is call eternity then?.

I really don't understand.

人生就是为了找寻爱的过程，每个人的人生都要找到四个人。
第一个是自己,
第二个是你最爱的人，
第三个是最爱你的人，
第四个是共度一生的人.
首先会遇到你最爱的人，然後体会到爱的感觉；
因为了解被爱的感觉，所以才能发现最爱你的人；
当你经历过爱人与被爱，学会了爱，才会知道什么是你需要的，
也才会找到最适合你，能够相处一辈子的人。
但很悲哀的，在现实生活中，这三个人通常不是同一个人；
你最爱的，往往没有选择你；
最爱你的，往往不是你最爱的；
而最长久的，偏偏不是你最爱也不是最爱你的，
只是在最适合的时间出现的那个人。
你，会是别人生命中的第几个人呢？
没有人是故意要变心的，他爱你的时候是真的爱你，
可是他不爱你的时候也是真的不爱你了，
他爱你的时候没有办法假装不爱你；
同样的，他不爱你的时候也没有办法假装爱你 。
当一个人不爱你要离开你，
你要问自己还爱不爱他，
如果你也不爱他了，千万别为了可怜的自尊而不肯离开；
如果你还爱他，你应该会希望他过得幸福快乐，
希望他跟真正爱的人在一起，绝不会阻止，
你要是阻止他得到真正的幸福，就表示你已经不爱他了，
而如果你不爱他，你又有什么资格指责他变心呢？
爱不是占有，
你喜欢月亮，不可能把月亮拿下来放在脸盆里，
月亮的光芒仍可照进你的房间。
换句话说，你爱一个人，也可以用另一种方式拥有，
让爱人成为生命里的永恒回忆，
如果你真爱一个人，就要爱他原来的样子─爱他的好，也爱他的坏：
爱他的优点，也爱他的缺点，
绝不能因为爱他，就希望他变成自己所希望的样子，
万一变不成就不爱他了。
真正爱一个人是无法说出原因的，
你只知道无论何时何地、心情好坏，你都希望这个人陪著你；
真正的感情是两人能在最艰苦中相守，也就是没有丝毫要求。
毕竟，感情必须付出，而不是只想获得；
分开是一种必然的考验，
如果你们感情不够稳固，只好认输，
真爱是不会变成怨恨的。
两人在谈情说爱的时候，
最喜欢叫对方发誓，许下承诺我们为什么要对方发誓，
就是因为我们不相信对方，我们根本不相信情人，
而这些山盟海誓又很不切实际：
海枯石烂、地老天荒，都不能改变我对你的爱!
明知道海不会枯、石不会烂、地不会老、天不会荒；
就算会，也活不到那时候。
许下诺言的时候千万注意，不要许下可以实现的诺言，
最好是承诺做不到的事，
反正做不到的，随便说说也不要紧，
请记住：”不可能实现的诺言最动人”
在爱情里，说的是一套，做的是另一套；
讲的人不相信，听的人也不相信。
你呢？找到了第几个？
茫茫人海中，你遇见了谁？谁又遇见了你？

I like this.
Copy and paste from other's blog.
Read this and perhaps you may be enlightened =)

I can be freaking weak emotionally,
but I can be strong mentally.

Cheers,
Hais.

do re me
My question to u,
Do U Really EXIST?.

Hokay, I succeed in forgetting you =)

Had so much talk today,
realized so much thing.

As what Kiwi had said,
never goes against the mother's nature.
He's absolutely right.
Thanks for the enlightenment man.

If you ever find a guy you like,
just speak it out,
it won't kill anyway.

But for my case,
too bad it fades away long time ago,
till now,
no interest in any man.
I really dunno why.

Trust is gone,
faith is lost.

Anyway, I wanna be happy =)

Certain things you just need not explain but it's there,
fixed.
If you ask why, there's no meaning anymore.

From here, I realized how much I treasure my friends and family,
if I ever lose them,
I might have died.
Maybe the reason for me to move on,
is to brighten up other's days and hence mine too.

From today onwards,
heart is locked,
key is gone.
I will move on for my dream,
a diploma first.
I shall make a close step towards hotel management.

Gambatte kudasai =D
Wahahas.

Hope ta cya soon!.

Cheers,
Manda

bleahs
Simply feels so weird today.
Aimm lab for merely an hour and break for a long time before PCT lect started.
Managed to finish CSAS draft and yah, hope Vigi won't be so fussy and make things hard for us -.-
And I'm really tired of editing my introduction again!.
Pls don't torture me again T.T
Hahahs.

Was in deep thoughts today.
Once I'm free, my imagination starts to run wild.
I can't help recalling, only way is to leave me alone and have a good rest.
Don't request for story here, cos I dun even have the contents here.
All I need is peace bah.

I think he's the only person I'd never even met and yet had some feelings for.
And I fell into his trap.
This shows manda is dumb.
Why things just don't go well for me?.
What a dilemma -.-
Confusion is all I have.

So near yet so far.
Only if you encounter the real situation, you'll know what substance is.

Hokay, let's not talk bout him anymore.
I ought to forget bout him.
Move on manda.

Yeah!.
Went for karate in the evening.
Then crap alot again.
I'm an insane today and yah rather than emo-ing?. lol
If I can make others laugh like hell, why not?.
I don't care what others think of me, just do what I desire.
JUST DO IT!.
Sensei was so humorous today lah.
Teaching and joking along...
Then he reminded us of the grading.
Omg!. It'll be held real soon!..
So scary and I really need to attend more trainings T.T
Simply not tough enough.
Wooosh!.


Hayashi rocks!.






Hokay, above are some movements or we call stunts we used in karate. Main purpose: To block and not to kill!. lol.
And yeah!. I love the last pict, only pict I can find in yahoo image XP
Sparring!. I hope it's spelled correctly -.-
Hahahs. Yah, I love kicking, I'm a violent ass!.
Hokay, I'm done for the day and gonna tuck in soon =)
Nites peeps!.
Oh yah, take good care of ya health too!. Woots.

Cheers,
Insane